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Pharmacokinetics – Summative Revision Notes

Morning Rounds · Pharmacokinetics · Summative Revision
Morning Rounds · Numerical Series · Pharmacokinetics
Pharmacokinetics
Summative Revision Notes
Seven rounds · NEET-PG / INI-CET / UPSC CMS · Formulae, thresholds, clinical anchors & traps
Zero & First Order Half-Life & Washout Vd & Loading Dose Clearance & Steady State TI · ED50 · LD50 Clinical Numericals Special Populations

These notes consolidate all seven Pharmacokinetics Morning Rounds. They are written for rapid pre-exam revision — not first-time learning. Each section heading links to its quiz. The formula reference blocks are printed in the format examiners expect. Every trap identified in the round debriefs appears here as a single-line recall badge.

The Core Distinction
FeatureZero-OrderFirst-Order
What is constant?Amount eliminated per unit timeFraction (%) eliminated per unit time
Rate depends on concentration?No — rate is fixedYes — rate falls as concentration falls
Half-life conceptDoes not apply (changes with dose)Constant; independent of dose
Plasma conc. vs time plotStraight line (linear)Exponential decay (log-linear straight)
Clinical examplesAlcohol, phenytoin (high dose), aspirin (OD)Most drugs at therapeutic doses
Key Formulae
First-order: Amount remaining after n half-lives = C₀ × (½)ⁿ % eliminated after n half-lives = 100 − [100 × (½)ⁿ] Zero-order: Amount remaining = Initial dose − (Rate × Time)
The Vd Analogy — Why It Matters

Vd is not a real anatomical volume. It is the hypothetical volume needed to contain all the drug in the body at the same concentration as measured in plasma. A drug with Vd = 700 L in a 70 kg person has not disappeared — it has been sequestered in tissues, leaving almost nothing in plasma. This is why haemodialysis, which filters only plasma, is futile for drugs with large Vd. The swimming pool analogy: dissolve a teaspoon of salt in a glass (high plasma concentration, small Vd) versus a swimming pool (undetectable in plasma, but the drug is still there — in tissues).

3 half-lives → 87.5% eliminated (not 75%) Zero-order: constant amount, not fraction Alcohol, phenytoin OD, aspirin OD = zero-order Most therapeutic drugs = first-order

Key Formulae
k (elimination rate constant) = 0.693 ÷ t½ Back-calculate t½: count the number of halvings in the interval, then t½ = Time ÷ n Time to steady state = 4–5 × t½ (independent of dose or dosing interval) Time to 95% elimination ≈ 5 × t½
The 4–5 Half-Life Rules
Half-lives elapsed% Eliminated% Remaining
150%50%
275%25%
387.5%12.5%
493.75%6.25%
596.875%~3%
Critical Distinction — PK Half-Life vs Clinical Duration

Half-life governs plasma concentration, not always duration of clinical effect. Phenelzine (MAOI) has a plasma t½ of ~2 hours but requires a 14-day washout before starting serotonergic drugs — because it irreversibly destroys MAO enzyme, and new enzyme synthesis takes 14 days. Being at steady state does not mean reaching a new steady state instantly after a dose change — re-equilibration always takes another 4–5 half-lives.

Steady state reached in 4–5 t½ regardless of dose 95% elimination ≈ 5 × t½ MAOI washout = enzyme resynthesis, not PK clearance k = 0.693 ÷ t½ — memorise this

Key Formulae
Vd = Dose ÷ C₀ (initial plasma concentration) IV Loading Dose = Target Cp × Vd Oral Loading Dose = (Target Cp × Vd) ÷ F (bioavailability)
Compartment Benchmarks — Memorise These Three
Vd valueDrug locationExample
~3–5 LConfined to plasmaHeparin, warfarin, monoclonal antibodies
~15 LExtracellular fluidGentamicin, mannitol
~42 LTotal body waterEthanol, theophylline
>100 LExtensive tissue sequestrationChloroquine (>200 L/kg), amiodarone (~5000 L), digoxin (~500 L)
Clinical Corollaries of Large Vd

Haemodialysis is futile for drugs with large Vd — dialysis filters plasma only. With Vd = 500 L (digoxin), less than 1% of total body drug is in plasma at any time. Loading doses are necessary when Vd is large AND half-life is long — waiting for steady state with amiodarone (t½ ~50 days, Vd ~5000 L) would take up to 275 days. The loading dose rapidly fills the large distribution volume to achieve therapeutic levels immediately.

Vd = Dose ÷ C₀ Oral LD = (Cp × Vd) ÷ F Large Vd → dialysis futile Large Vd + long t½ → loading dose needed Benchmarks: 3 L plasma, 15 L ECF, 42 L TBW

Key Formulae
Css = Infusion rate ÷ Clearance  [UNITS MUST MATCH] CL = 0.693 × Vd ÷ t½  (or CL = k × Vd) Maintenance dose = Css × CL ÷ F Renal dose adjustment: New dose = Normal dose × (Patient CrCl ÷ Normal CrCl)
The Units Trap — The Most Common Exam Error

Infusion rate is given in mg/hour. Clearance is given in mL/min. You cannot divide directly. Convert first:

mL/min × 60 = mL/hour → ÷ 1000 = L/hour

Example: clearance 100 mL/min = 6000 mL/hr = 6 L/hr. Infusion rate 200 mg/hr ÷ 6 L/hr = 33.33 mg/L. The distractor 50 mg/L comes from dividing by 4 (using 100 mL/min as if it were L/hr).

Hepatic Extraction Ratio — Flow vs Capacity
Extraction ratioClearance depends onEffect of cirrhosisExamples
High (>0.7)Liver blood flowDramatic rise in oral bioavailability; toxicity on normal dosesPropranolol, morphine, lignocaine, labetalol
Low (<0.3)Enzyme capacityModest effect on bioavailabilityWarfarin, diazepam, theophylline

Css = Rate ÷ CL — convert units first CL = 0.693 × Vd ÷ t½ High extraction drugs dangerous in cirrhosis New steady state after rate change = another 4–5 t½ mL/min × 60 ÷ 1000 = L/hr

Key Formulae
Therapeutic Index (TI) = LD50 ÷ ED50  [higher = safer] Certain Safety Factor (CSF) = TD1 ÷ ED99  [more clinically honest]
TI Interpretation Anchors
TI valueInterpretationExamples
>100Very wide safety marginPenicillin, paracetamol (therapeutic range)
~10Reasonable marginMost antihypertensives
<2Narrow therapeutic index (NTI) — requires monitoringDigoxin, lithium, phenytoin, warfarin, theophylline, aminoglycosides
Potency vs Efficacy — The Distinction Examiners Exploit

Potency = dose required for a given effect. Lower ED50 = more potent. Efficacy = maximum effect a drug can produce. These are independent axes. A more potent drug is not safer and not more efficacious. Botulinum toxin is the most potent substance known — its margin to lethality is correspondingly tiny. Codeine and morphine have the same ceiling effect (efficacy); morphine is simply more potent.

Why CSF Matters More Than TI

Classic TI uses median values — the middle of the population. CSF asks what happens when you give the dose needed to treat the most resistant 1% of patients (ED99) to the most sensitive 1% (TD1). A CSF of 2 means these two curves nearly overlap. Digoxin's CSF approaches 1 — explaining why routine therapeutic monitoring is mandatory.

TI = LD50 ÷ ED50 (not inverted) NTI drugs: digoxin, lithium, phenytoin, warfarin, theophylline, aminoglycosides Potency ≠ safety ≠ efficacy CSF = TD1 ÷ ED99 Higher TI = wider safety margin

High-Yield Clinical Integrations
Drug / ScenarioKey calculationClinical lesson
Digoxin toxicityTotal body burden = Cp × Vd
(4 ng/mL × 500 L = 2000 mcg)
<1% in plasma → haemodialysis futile → use Digibind
Theophylline maintenanceDose = Css × CL ÷ F
(convert mL/min → L/hr first)
Units trap: 40 mL/min = 2.4 L/hr, not 40
Phenytoin dose increase33% dose rise → 200% Cp riseMichaelis-Menten saturation: enzymes near-saturated at therapeutic levels
Thiopentone offsetWakes in 10 min; t½ = 11 hoursRedistribution from brain to muscle/fat — not elimination
Bioavailability from AUCF = (AUCoral/Doseoral) ÷ (AUCIV/DoseIV)Dose-normalise before dividing — the most common error
Michaelis-Menten (Saturation) Kinetics

At low concentrations: first-order behaviour (enzymes not saturated). At high concentrations: zero-order behaviour (enzymes saturated). Phenytoin operates at the inflection point near therapeutic levels — small dose increases produce disproportionately large rises in plasma concentration. Clinical rule: adjust phenytoin in 25–50 mg increments only and recheck levels after 2 weeks. Other MM drugs: aspirin (high dose), alcohol, salicylates.

Two-Compartment Kinetics — Redistribution

Thiopentone: highly lipophilic, rapidly enters brain (high blood flow) → unconsciousness. Then redistributes to muscle and fat (large mass) → brain concentration falls below anaesthetic threshold → patient wakes. Total body drug: barely changed. Terminal t½ of 11 hours reflects slow release from fat + hepatic metabolism, not the clinical offset. Same principle applies to diazepam single-dose clinical duration.

Total drug = Cp × Vd Digoxin toxicity → Digibind, not dialysis Phenytoin: MM kinetics, adjust in 25–50 mg steps Thiopentone offset = redistribution, not elimination Bioavailability: dose-normalise AUCs before ratio

Bioavailability & First-Pass — Route Anatomy

Oral route: gut → portal vein → liver → first-pass metabolism → systemic circulation. Sublingual: superior vena cava → systemic circulation directly (bypasses liver). GTN oral bioavailability <1%; sublingual ~80%. If a patient swallows a sublingual GTN tablet — no therapeutic effect. Transdermal and IV routes also bypass first-pass.

Protein Binding — Free Drug Principle

Only free (unbound) drug is pharmacologically active, distributes across membranes, and is available for elimination. Bound drug is inert reservoir. Warfarin 99% bound: displacing 1% of binding sites doubles the free fraction from 1% to 2% — doubling pharmacological effect on the same total dose. High-risk displacing drugs: phenylbutazone, aspirin, NSAIDs, sulfonamides.

Special Populations — Rapid Reference
PopulationKey PK changeDosing implication
NeonatesGFR ~30% adult; immature CYP450; glucuronidation deficient; TBW 80%Extend dosing intervals; higher mg/kg Vd for water-soluble drugs; avoid chloramphenicol (grey baby syndrome)
ElderlyReduced muscle mass → low creatinine production → serum Cr appears normal despite reduced GFRAlways calculate CrCl (Cockcroft-Gault); never trust serum creatinine alone
PregnancyPlasma volume +40%; albumin −20%; GFR increases; hepatic CYP activity changesExpanded Vd dilutes drug; reduced albumin raises free fraction; NTI drugs need monitoring throughout
Hepatic failureHigh-extraction drugs: dramatically increased oral bioavailability (portosystemic shunting)Propranolol, morphine, lignocaine — reduce dose substantially or avoid
Renal failureClearance of renally-excreted drugs falls proportionally with GFRReduce dose OR extend interval; both strategies are pharmacokinetically valid
The Elderly Creatinine Trap

Sarcopenia (reduced muscle mass) → less creatinine produced → serum creatinine appears "normal" despite severely impaired GFR. Cockcroft-Gault must be calculated in all elderly patients before prescribing renally-cleared drugs. A serum creatinine of 90 µmol/L in an 80-year-old woman of 45 kg may correspond to a CrCl of only 22 mL/min — severe renal impairment requiring significant gentamicin dose reduction and interval extension.

Swallowed GTN → <1% bioavailability → no effect Free drug = active drug Warfarin displacement: 1% → 2% free = double effect Elderly: calculate CrCl, never rely on serum Cr alone Neonates: extend intervals; immature CYP + GFR Pregnancy: monitor NTI drugs throughout

Cross-Series · Pharmacokinetics
Examiner's Favourites — Rapid Recall
Formula reference — one line each
FormulaExpression
VdDose ÷ C₀
0.693 × Vd ÷ CL
CL (total body)0.693 × Vd ÷ t½
Css (infusion)Infusion rate ÷ CL  [units must match]
Loading dose (IV)Target Cp × Vd
Loading dose (oral)(Target Cp × Vd) ÷ F
Maintenance doseCss × CL ÷ F
Bioavailability (F)(AUCoral/Doseoral) ÷ (AUCIV/DoseIV)
Therapeutic IndexLD50 ÷ ED50
Certain Safety FactorTD1 ÷ ED99
k (rate constant)0.693 ÷ t½
Unit conversion anchors — do these before every calculation

mL/min → L/hr: × 60 then ÷ 1000 ng/mL = mcg/L (equivalent units) mL × concentration → ng or mcg (watch the prefix) Always match time units (hr vs min) before dividing

Drugs with notable pharmacokinetics — exam anchors

Digoxin: Vd 500 L, t½ 36 hr, NTI — Digibind for toxicity Amiodarone: Vd ~5000 L, t½ 40–55 days — loading dose mandatory Chloroquine: Vd >200 L/kg — dialysis futile in OD Phenytoin: MM kinetics — 25–50 mg increments only GTN: oral bioavailability <1% — sublingual/IV/transdermal only Propranolol/morphine/lignocaine: high extraction — dangerous in cirrhosis Thiopentone: offset by redistribution to fat, not elimination Warfarin 99% bound: displacement doubles free fraction

Sequence rules — act in order

Convert units → apply formula → interpret clinically Dose-normalise AUCs before calculating bioavailability Calculate CrCl before prescribing in the elderly — serum Cr alone is unreliable Adjust phenytoin in small increments, recheck after 2 weeks In overdose: calculate Vd before deciding on dialysis In loading dose: remember to divide by F for oral route

The traps examiners set repeatedly

mL/min ≠ L/hr — convert before dividing for Css 87.5% eliminated at 3 half-lives, not 75% TI = LD50 ÷ ED50 (not inverted, not subtracted) Potency ≠ safety ≠ efficacy — three independent properties Serum Cr "normal" in elderly ≠ normal renal function Doubling infusion rate doubles Css, but new SS still takes 4–5 t½ MAOI washout = enzyme resynthesis (14 days), not PK clearance (2 hrs) Dose-normalise AUC before bioavailability ratio — not raw AUC

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